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RF-O2熒光光纖氧氣測量技術——氧氣測量全面解決方案

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RF-O2熒光光纖氧氣測量技術是基于REDFLASH光極傳感器技的氧氣測量技術記得牢,由歐洲Pyroscience公司及Graz大學等科學家研制生產註入了新的力量,由光極氧氣傳感器、測量儀及軟件組成更多可能性,廣泛應用于環(huán)境科學去創新、生態(tài)科學積極回應、植物科學、動物科學又進了一步、海洋科學多種場景、生物醫(yī)學、生物技術規劃、食品科學等各個領域擴大公共數據,其主要功能特點如下

1) REDFLASH光極氧氣傳感器技術,高精確度帶動擴大、高穩(wěn)定性核心技術體系、高時空解析度、低能耗核心技術、無耗氧應用提升、無交叉敏感性

2) 傳感器類型靈活多樣,有探頭式創造性、探針式發展的關鍵、非接觸式(sensor spot)及納米微粒式等,適應于液體和氣體不同條件下的O2測量

3) 有內置sensor spot的流通管和呼吸瓶規模設備,非接觸式測量流動液體的溶解氧及呼吸瓶內液體或氣體中氧氣含量

4) 輕便緊湊型FireStingO2測量儀真諦所在,內置水汽、氣壓傳感器競爭力,有1充分、2、4通道供選配集聚,可分別接1個競爭力、2個、4個光極氧氣傳感器狀況,另有Mini型FireStingO2-mini供選配

5) U盤式PiccolO2測量儀——世界上小的O2測量儀機製性梗阻,可連接一個O2傳感器,USB口連接電腦同期,即插即用

圖片63.png

測量原理:

REDFLASH光極O2傳感器技術生產效率,利用*的O2敏感REDFLASH指示劑,通過610-630nm調制紅光激發(fā)效果,REDFLASH指示劑發(fā)出760-790nm紅外熒光使用,熒光強度隨接觸的O2分子濃度升高而發(fā)生熒光淬滅,這種熒光動態(tài)通過光纖傳輸?shù)綔y量儀密度增加,測量儀靈敏地檢測其相位漂移并據(jù)此換算成O2濃度有效性。

應用領域:

1) 水體溶解氧測量監(jiān)測、藻類及藻類生物膜光合作用與呼吸作用測量監(jiān)測

2) 植物光合作用與呼吸作用測量監(jiān)測

3) 水生動物(魚類機遇與挑戰、水生昆蟲等無脊椎動物廣泛關註、浮游動物等呼吸代謝測量

4) 陸生動物善於監督、實驗動物、動物組織就能壓製、血液等呼吸代謝測量

5) 土壤更合理、濕地、海洋沉積更優美、河湖沉積剖面O2測量

6) 生物反應器實際需求、發(fā)酵過程、酶動力學優勢、細胞培養(yǎng)等O2測量監(jiān)測

7) 糧食食品儲運善謀新篇、葡萄酒等O2測量監(jiān)測

8) 污水處理、沼氣便利性、垃圾填埋場方法、有機物降解等O2測量監(jiān)測

技術指標:

1) FireStingO2FSO2)測量儀:

a) 1通道、2通道提供有力支撐、4通道可供選配切實把製度,分別可接1個、2個和4個O2傳感器最深厚的底氣,可并聯(lián)組成8通道甚至更多通道協同控製;另具備一個溫度傳感器通道(可選配4通道溫度傳感器)圖片66.pngb) 激發(fā)光源620nm,監(jiān)測器760nm(NIR)

c) 采樣頻率:每秒4次

d) 內置氣壓傳感器品質,300-1100mbar,0.06mbar分辨率深入各系統,精確度±3mbar

e) 內置濕度傳感器解決問題,0-*,分辨率0.04%作用,精確度±0.2%

f) 內置溫度傳感器相互配合,-40125°C,分辨率0.01°C著力增加,精確度±0.3°C

g) 具模擬輸出和自動模式智能化,0-2.5VDC

h) USB接口,通過USB口PC供電

i) 大刑幚?。?/span>68x120x30mm建設,重350g

2) PiccolO2 U盤式測量儀:大小僅15x15x54mm,重量約20g助力各行,單通道前來體驗,激發(fā)光620nm,檢測器760nm確定性,采樣頻率每秒20次更加廣闊p耗?刹⒙?lián)組成多通道測量系統(tǒng)”3址€定?赏ㄟ^PiccoTHP測量溫濕度和氣壓并進行補償

3) 探頭式O2傳感器:直徑3mm總之,測量范圍0-50%(0-23mg/l)(可選配其它范圍),檢測極限0.02%(0.01mg/l)支撐作用,分辨率0.05%(0.025mg/l)@20% O2工藝技術,精確度±0.2%(0.1mg/l)@20% O2,低使用壽命1千萬數(shù)據(jù)點規模,存儲時間大于3年(室溫暗處儲放)

圖片69.png

4) 探針式O2傳感器:有固定探針式近年來、可伸縮探針式、尖頭式及圓頭式等不同類型供選配發展目標奮鬥;探針直徑有50μm技術先進、230μm430μm等規(guī)格延伸;測量范圍0-50%(0-23mg/l)(可選配其它范圍)認為,檢測極限0.02%(0.01mg/l),分辨率0.05%(0.025mg/l)@20% O2新趨勢,精確度±0.2%(0.1mg/l)@20% O2反應能力,響應時間小于1s(與探針粗細有關),低使用壽命1百萬數(shù)據(jù)點學習,存儲時間大于3年(室溫暗處儲放)

5) 非接觸式(sensor spot)O2傳感器(見下左圖):用于非接觸性測量監(jiān)測透明容器中的氧氣含量結構重塑,傳感器貼用硅膠等貼附在容器內壁,通過固定在外壁的光纖將熒光動態(tài)信號傳輸?shù)綔y量儀以檢測O2濃度應用優勢;測量范圍0-50%(0-23mg/l)(可選配其它范圍)高質量發展,檢測極限0.02%(0.01mg/l),分辨率0.05%(0.025mg/l)@20% O2高效節能,精確度±0.2%(0.1mg/l)@20% O2影響力範圍,低使用壽命2千萬數(shù)據(jù)點,存儲時間大于3年(室溫暗處儲放)

6) 納米微粒傳感器(參見上右圖):納米技術新創新即將到來,用于非接觸性測量微量液體中O2含量邁出了重要的一步,即時響應,測量范圍0-50%(0-23mg/l)設施,檢測極限0.02%(0.01mg/l)需求,分辨率0.05%(0.025mg/l)@20% O2,存儲時間大于3年(室溫暗處儲放)

7) 流通管:內置非接觸式O2傳感器規模設備,用于流動液體O2測量監(jiān)測(如魚類呼吸代謝測量等)真諦所在,測量范圍0-50%(0-23mg/l)(可選配其它范圍),檢測極限0.02%(0.01mg/l)技術創新,分辨率0.05%(0.025mg/l)@20% O2深入交流研討,精確度±0.2%(0.1mg/l)@20% O2資料,低使用壽命1千萬數(shù)據(jù)點,存儲時間大于3年(室溫暗處儲放)

圖片74.png

8) 呼吸瓶:內置非接觸式O2傳感器關註度,用于生物呼吸測量(如藻類橫向協同、小型魚類、魚卵敢於挑戰、昆蟲等)不斷創新,標準配置有4ml和20ml兩種規(guī)格

9) Pyro Oxygen Logger軟件用于參數(shù)設置、校準提供了遵循、數(shù)據(jù)顯示包括圖表顯示參與水平、數(shù)據(jù)輸出等功能

圖片75.png

應用案例:

案例1:法國Bordeaux大學利用FSO2 4通道熒光光纖氧氣測量儀,對Aquitaine海岸沉積樣芯耗氧進行了測量分析服務效率,以研究海洋底棲動物活動(bioirrigation)對海岸帶生態(tài)系統(tǒng)生態(tài)過程及生物地理化學功能(如沉積有機物的再礦化)的影響明確相關要求。

案例2:芬蘭Turku大學利用FSO2和430μm光極氧探針,對南瓜類囊體懸浮液光合放氧進行了測量分析統籌發展。

案例3:美國Woods Hole海洋學研究所深化涉外,利用RF-O2非接觸式光極氧氣傳感器(sensor spot),對海洋無脊椎動物呼吸代謝進行了測量分析生產製造,以研究其固有的生物鐘與環(huán)境脅迫的關系開展試點,這些海洋無脊椎動物體重只有0.5-50mg。圖中為翼足類軟體動物在不同濃度CO2條件下的耗氧率共同。

圖片76.png

案例4:澳大利亞海洋科學研究所推進一步、瑞典Gothenburg大學等組成的科學小組,利用Pyroscience的REDFLASH氧氣測量技術強大的功能,對河鱸(Perca fluviatilis)呼吸代謝進行測量分析實際需求,以研究其熱耐受性和適應性的生理機制。他們選擇波羅的海核電站附近的一個瀉湖優勢,核電站排出的熱水進入該瀉湖,在過去30年大量魚類因為不適應水溫升高而滅絕增產,但河鱸卻得以繁盛便利性,該地成為理想的研究氣候變暖對魚類種群影響的“天然實驗室”。他們測量河鱸呼吸代謝率的同時行動力,還測量其靜脈血液在溫度升高狀態(tài)下的氧分壓提供有力支撐,靜脈血是河鱸心臟供氧的主要來源,高溫條件下靜脈血氧氣含量被認為是其心臟功能的重要限制因子良好。

圖片77.png

案例5:德國Ulm大學利用FSO2測量儀和50μm可伸縮式RFO2探針逐步顯現,對患者腦脊髓液(CSF)樣品溶解氧進行測量分析,以研究探討神經紊亂及神經炎等疾病的生理和診斷引領。

案例6:德國農業(yè)科學與景觀研究機構自動化裝置,利用FSO2測量儀和RFO2探針示範,對土壤氧氣進行測量,以評估不同種類蚯蚓在低氧條件下對土壤改良的效率有很大提升空間。

案例7:西班牙Valladolid大學利用RFO2熒光光纖氧氣測量技術運行好,監(jiān)測葡萄酒橡木桶O2吸收——對葡萄酒品質至關重要但一直以來缺乏科學的了解。葡萄酒在橡木桶內(3-24個月)的過程溶解氧至關重要可能性更大,因為O2調節(jié)了葡萄酒整個的熟化過程部署安排。

近期部分參考文獻:

2015

1. Experimental manipulations of tissue oxygen supply do not affect warming tolerance of European perch. Brijs et al., 2015, J Exp Biol, in press

2. The formation of aggregates in coral reef waters under elevated concentrations of dissolved inorganic and organic carbon: A mesocosm approach. Cárdenas et al., 2015, Mar Chem, in press

3. Efficient gas–liquid contact using microfluidic membrane devices with staggered herringbone mixers. Femmer et al., 2015, Lab on a Chip: DOI: 10.1039/C5LC00428D

4. Three-dimensional structure and cyanobacterial activity within a desert biological soil crustRaanan et al., 2015, Environ Microbiol: doi:10.1111/1462-2920.12859

5. Photoacoustic lifetime imaging for direct in vivo tissue oxygen monitoringShao, Q. & Ashkenazi, S., 2015, J Biomed Optics 20(3): doi:10.1117/1.JBO.20.3.036004

6. Laccase mediated oxidation of industrial lignins: Is oxygen limiting?Ortner et al., 2015, Process Biochem Vol 50 (8): 1277-1283

7. Increased gastrointestinal blood flow: An essential circulatory modification for euryhaline rainbow trout (Oncorhynchus mykiss) migrating to seaBrijs et al., 2015, Scientific Reports 5, Article number:10430: doi:10.1038/srep10430

8. Not so monofunctional—a case of thermostable Thermobifida fusca catalase with peroxidase activityLoncar, N. & Fraaije, M.W., 2015, Appl Microbiol Biotechnol Vol 99 (5): 2225-2232

9. An Assessment of the Precision and Confidence of Aquatic Eddy Correlation MeasurementsDonis et al., 2015, J Atmos Oceanic Technol 32 (3): 642–655

10. Pharmaceuticals and personal care products alter growth and function in lentic biofilmsShaw et al., 2015, Environ Chem 12(3): 301-306

11. Futile cycling increases sensitivity toward oxidative stress in Escherichia coli. Adolfsen K.J & Brynildsen M.P., 2015, Metabolic Engin Vol 29: 26-35

12. Accumulation of Basic Amino Acids at Mitochondria Dictates the Cytotoxicity of Aberrant UbiquitinBraun et al., 2015, Cell Reports Vol 10 (9): 1557-1571

13. O2 mass transfer in an oscillatory flow reactor provided with smooth periodic constrictions. Individual characterization of kL and a. Ferreira et al., 2015, Chem Eng J Vol 262: 499-508

14. Flexibility in metabolic rate confers a growth advantage under changing food availabilityAuer et al., 2015, J Animal Ecol: doi: 10.1111/1365-2656.12384

15. Oxygen metabolism and pH in coastal ecosystems: Eddy Covariance Hydrogen ion and Oxygen Exchange System (ECHOES)Long et al., 2015, Limnol Oceanogr: Methods, DOI: 10.1002/lom3.10038

2014

1. Ocean acidification rapidly reduces dinitrogen fixation associated with the hermatypic coral Seriatopora hystrix. R?decker et al., 2014, Mar Ecol Progr Ser Vol 511: 297-302

2. All puffed out: do pufferfish hold their breath while inflated McGee, G.E. & Clark, T.D., 2014, Biol Lett Vol 10:

3. Spectral Effects on Symbiodinium Photobiology Studied with a Programmable Light Engine. Wangpraseurt et al., 2014, PLOS One 9: e112809.

4. The energetic cost of foraging explains growth anomalies in tadpoles exposed to predatorsBarry, M.J., 2014, Physiol Biochem Zool Vol 87: 829-836

5. A product of its environment: the epaulette shark (Hemiscyllium ocellatum) exhibits physiological tolerance to elevated environmental CO2Heinrich et al., 2014, Conserv Physiol Vol 2 (1): doi: 10.1093/conphys/cou047

6. Oxygen-Dependent Control of Respiratory Nitrate Reduction in Mycelium of Streptomyces coelicolor A3(2).Fischer et al., 2014, J Bacteriol Vol 196 (23): 4152-4162

7. A respiratory nitrate reductase active exclusively in resting spores of the obligate aerobe Streptomyces coelicolor A3(2)Fischer et al., 2014, Mol Microbiol Vol 89 (6):1259-73

8. Growth trajectory influences temperature preference in fish through an effect on metabolic rateKillen, S., 2014, J Animal Ecol Vol 83 (6): 1513-1522

9. Colored ceramic foams with tailored pore size and surface functionalization used as spawning plates for fish breedingKroll et al., 2014, Ceramics International Vol. 40 (10): 15763-15773

10. Aerobic scope predicts dominance during early life in a tropical damselfishKillen et al., 2014, Functional Ecol Vol 28 (6): 1367-1376

11. European sea bass, Dicentrarchus labrax, in a changing oceanPope et al., 2014, Biogeosciences Vol 11: 2519-2530

12. Marine rust tubercles harbour iron corroding archaea and sulphate reducing bacteriaUsher et al., 2014, Corrosion Science Vol 83: 189-197

13. Magnetic optical sensor particles: a flexible analytical tool for microfluidic devices. Ungerb?ck et al., 2014, Analyst Vol 139: 2551-2559

14. Investigation and correction of the interference of ethanol, sugar and phenols on dissolved oxygen measurement in wineAlamo-Sanza et al., 2014, Anal Chim Acta Vol 809: 162-173

15. Bioresponsive polymers for the detection of bacterial contaminations in plaet concentratesGamerith et al., 2014, New Biotechnol Vol 31 (2): 150-155

16. Life on the edge: thermal optima for aerobic scope of equatorial reef fishes are close to current day temperaturesRummer & Couturier, 2014, Global Change Biol Vol 20 (4): 1055-1066

17. The effect of diel temperature and light cycles on the growth of Nannochloropsis oculata in a photobioreactor matrix. Tamburic et al., 2014, PLOS One, DOI: 10.1371/journal.pone.0086047

18. Radiative energy budget reveals high photosynthetic efficiency in symbiont-bearing coralsBrodersen et al., 2014, J R Soc Interface Vol 11 (93), DOI: 10.1098/ rsif.2013.0997

19. The isotope effect of denitrification in permeable sediments. Kessler et al., 2014, Geochim Cosmochim Acta Vol 133: 156-167

20. Discovery and characterization of a 5-Hydroxymethylfurfural oxidase from Methylovorus sp. Strain MP688Dijkman & Fraaije, 2014, Appl Environ Microbiol Vol 80 (3): 1082-1090

21. Amperometric glucose sensing with polyaniline/poly(acrylic acid) composite film bearing covalently-immobilized glucose oxidase: A novel method combining enzymatic glucose oxidation and cathodic O2 reduction. Homma et al., 2014, J Electroanal Chem Vol 712: 119-123

22. C*tion and isolation of N2-fixing bacteria from suboxic waters in the Baltic SeaBentzon-Tilia et al., 2014, FEMS Microbiol Ecol Vol 88 (2): 358-371

23. Coenzyme regeneration catalyzed by NADH oxidase from Lactococcus lactis. Sudar et al., 2014, Biochem Engin J Vol 88: 12-18

24. Temporary storage or permanent removal? The division of nitrogen between biotic assimilation and denitrification in stormwater biofiltration systemsPayne et al., 2014, PLOS One, DOI: 10.1371/journal.pone.0090890

25. Increased rates of dissimilatory nitrate reduction to ammonium (DNRA) under oxic conditions in a periodically hypoxic estuaryRoberts et al., 2014, Geochim Cosmochim Acta Vol 133: 313-324

26. Compartmentalized microbial composition, oxygen gradients and nitrogen fixation in the gut of Odontotaenius disjunctus. Ceja-Navarro et al., 2014, The ISME J Vol 8: 6-18

27. Optimum temperatures for growth and feed conversion in cultured hapuku (Polyprion oxygeneios) – Is there a link to aerobic metabolic scope and final temperature preference?Khan et al., 2014, Aquaculture Vol 430: 107-113

28. Aerobic scope does not predict the performance of a tropical eurythermal fish at elevated temperaturesNorin et al., 2014, J Exp Biol Vol 217: 244-251

29. Aquatic Eddy Correlation: Quantifying the Artificial Flux Caused by Stirring-Sensitive O2 SensorsHoltappels et al., 2015, PLoS ONE 10(1):e0116564. doi:10.1371/journal.pone.0116564

30. Decreased light availability can amplify negative impacts of ocean acidification on calcifying coral reef organismsVogel et al., 2015, Mar Ecol Progr Ser Vol 521: 49-61

31. Physiological and ecological performance differs in four coral taxa at a volcanic carbon dioxide seepStrahl et al., 2015, Comp Biochem Physiol, Part A Vol 184: 179-186

32. Novel use of a micro-optode in overcoming the negative influence of the amperometric micro-probe on localized corrosion measurementsTaryba et al., 2015, Corrosion Science, accepted

33. The effect of temperature and ration size on specific dynamic action and production performance in juvenile hapuku (Polyprion oxygeneios)Khan et al., Aquaculture Vol 437: 67-74

34. The effect of temperature and body size on metabolic scope of activity in juvenile Atlantic cod Gadus morhua LTirsgaard et al., 2015, Comp Biochem & Physiol Part A: Mol & Integr Physiol Vol 179: 89-94



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